Device
pBAD-CDE-D

Part:BBa_K323132:Design

Designed by: Tina Lebar, Tjaša Stošicki   Group: iGEM10_Slovenia   (2010-10-20)

VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3078
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 4320
    Illegal SapI.rc site found at 4395


Design Notes

Part:BBa_K323087 was ligated into a vector containing Part:BBa_K323086 as a back insert. Part:BBa_K323131 was later ligated into the resulting BioBrick as a back insert. The fragment containing the latter three BioBrick parts was then ligated into the BsaI cloning site of Part:BBa_K323110.

Source

The part was designed from four parts (Part:BBa_K323110, Part:BBa_K323087, Part:BBa_K323086 and Part:BBa_K323131) that our team previously constructed.

References

Balibar CJ, Walsh CT. 2006. In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum. Biochemistry, 45:15444-57

Duran N, Justo GZ, Ferreira CV, Melo PS, Cordi L, Martins D. 2007. Violacein: Properties and biological activities. Biotechnology and Applied Biochemistry, 48: 127-133

Jiang P, Wang H., Zhang C, Lou K, Xing XH. 2010. Reconstruction of the violacein bioynthetic pathway from Duganella sp. B2 in different heterologous hosts. Applied Microbiology and Biotechnology, 86: 1077-1088

Christy B, Nathans D. 1989. DNA binding site of the growth factor-inducible protein Zif268. Proceedings of the National Academy of Sciences, 22: 8737–41

Kinzler KW, Vogelstein B. 1990. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Molecular and Cell Biology, 2: 634–42

Reynolds L, Ullman C, Moore M, Isalan M, West MJ, Clapham P, Klug A, Choo Y. 2003. Repression of the HIV-1 5LTR promoter and inhibition of HIV-1 replication by using engineered zinc-finger transcription factors. Proceedings of the National Academy of Sciences, 4:1615–1620

Ooi AT, Stains CI, Ghosh I, Segal DJ. Sequence enabled Reassembly of beta-lactamase (SEER-LAC) - A Sensitive Method for the Detection of Double-Stranded DNA.

Libri V, Onori A, Fanciulli M, Passananti C, Corbi N. 2004. The artificial zinc finger protein "Blues" binds the enhancer of the fibroblast growth factoer 4 and represses transcription. FEBS Letters, 560: 75-80